Young, 4-6 months old zebrafish aging, 19-23 months old zebrafish NC, negative control (no primary antibody) Rat, rat retina positive control Opa1-L, Opa1 long segment Opa1-S, Opa1 short segment GCL, ganglion cell layer. All photographs were taken at 40x magnification except rat sections were at 20x magnification. (H) Immunofluorescence localization and relative expression of Mfn2 in the RGC layer of young and aging zebrafish retina cross-sections. ( G) The ratio of Opa1-L/Opa1-S, as determined by densitometry of western blots as in E (*P<0.05, n=3. ( F) The graph depicts the densitometric mean and SEM normalized to the corresponding level of the loading control protein beta-tubulin (*P<0.05, n=3). In contrast to humans, zebrafish have extraordinary regeneration capacities of complex organs including the heart. Cardiovascular dysfunction has an increasing prevalence with age and is one of the leading causes of death. ( E) Representative western blot showing the protein expression levels of Mfn2 and Opa1 in young and aging zebrafish retinas. Abstract Age-associated organ failure and degenerative diseases have a major impact on human health. ( A-D) Mfn2, Opa1, Oma1, and Fis1 expression in young and aging zebrafish retinas. Young, 4-6 months old zebrafish aging, 19-23 months old zebrafish NC, negative control (no primary antibody) Rat, rat retina positive control Opa1-L, Opa1 long segment Opa1-S, Opa1 short segment GCL, ganglion cell layer. Publication types Research Support, Non-U.S. Thus, zebrafish scale represents a useful model for analyzing the osteoblast behaviour during bone formation and mineralization and it could be useful in physiological studies and pharmacological tests. Mitochondrial fusion/fission imbalanced in aging zebrafish retinas. The internal circuli are also characterized by new matrix deposition.
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